The present study was undertaken to quantify the total polyphenolic and flavoniod content of mulberry leaf obtained from three popular cultivars viz., Goshoerami (Morus multicaulis), Ichinose (Morus Alba) and Koksu-21 (Morus Alba) and measure their corresponding antioxidant properties/ scavenging activity. The ethanol stirrer extraction method was used for the preparation of mulberry leaf extracts. The leaf extract of Koksu-21(tender) and Ichinose (tender) recorded the highest total polyphenolic and flavoniod contents to the tone of 8.58mgGAE/g and 73.03 mgQE/g of dry weight respectively. Further, significantly higher (21.20%) scavenging activity on DPPH was observed in the Ichinose (tender) leaf extract. The study has suggested that mulberry leaf, can serve as a promising source of dietary antioxidants and further purification of these identified compounds will prove to be one of the important sources for pharmaceutical and nutraceuticals applications and would thereby add value to sericulture industry.