author(s)
Akshaya SB, Krishnamoorthy AS, Nakkeeran S, Poornima K and Sivakumar U

abstract
The present study focused on the nematicidal activities of bio-molecules extracted from mushrooms against M. incognita. The experimental results revealed that the highest hatching inhibition and the juvenile mortality were recorded with the ethyl acetate fraction of cell-free culture (CFC) filtrate of Ganoderma lucidum followed by Lentinus edodes. At 1000 ppm concentration, the bio-active molecules of G. lucidum exhibited the maximum inhibition of egg hatching (92.6%) and juvenile mortality (93.2%) of M. incognita at 72 hours of incubation. GC –MS analysis of G. lucidum revealed the presence of 23 compounds viz. ., Octadecane,3-ethyl-5-(2-ethyl butyl), Decane, Undecane, 3,7-dimethyl-, Dihydroartemisinin, Benzaldehyde, 3,4-dimethyl-, Heptadecane, 2,6,10,15-tetramethyl-, 2,4-Di-tert-butyl-phenol, 1-Propanamine, Pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-,1,2-Benzenedicarboxylic acid, butyl octyl ester, n-Hexadecanoic acid, Dibutyl phthalate, 2,2,4-Trimethyl-3-(3,8,12,16-tetramethylheptadeca-3,7,11,15-tetraenyl)-cyclohexanol, Methyl stearate, Cyclohexane, Octadecanoic acid, Deoxyspergualin, Methyl glycocholate, 3TMS derivative, 1,25-dihydroxyvitamin D3, TMS derivative, Spirost-8-en-11-one, 3-hydroxy-, 1H-Indene, 1-hexadecyl-2,3-dihydro- and Bis(2-Ethylhexyl) phthalate. Among these, 2, 4-Di-tert-butyl-phenol, n-Hexadecanoic acid and Dibutyl phthalate might have been responsible for antinemic activity.