author(s)
A Palanisammi, S Satheshkumar and S Rangasamy

abstract
Murrah graded pluriparous buffaloes (n=10) were subjected for three superovulatory treatments. Treatment I (Control; n=20): Follicle Stimulating Hormone (600 mg) was initiated on the Day 10 for a period of five days. Treatment II (SO-OS; n = 7): Superstimulatory treatment was initiated after sychnronization of oestrous cycle by Ovsynch protocol. Gonadotrophin treatment for superovulation was initiated on Day 6 of synchronized cycle. Treatment III (SO-FWS; n = 7): GnRH (Inj. Receptal; 10 µg i.m.) was administered to all the animals on Day 6 of the cycle (Day 0: natural oestrus) and FSH treatment was initiated 96 h after GnRH (Day 10). Animals were inseminated thrice with frozen thawed proven during superovulatory oestrum and embryos/ova were recovered non-surgically on Day 5.5 of the superovulatory cycle. Six animals (30.0%) in control group did not respond for the treatment, while all the animals (100%) in SO-OS and SO-FWS groups responded for superovulation treatment. The mean number of CL is significantly (P < 0.05) higher in SO-OS group than the other groups. The control and SO-FWS groups had significantly (P < 0.01) greater number of anovulatory follicles (AFs). More percentage of transferrable quality embryos were recovered from SO-OS group, while more number of unfertilized ova and poor quality embryos were recovered from control and SO-FWS groups. It could be concluded that superovulatory response, embryo yield and embryo quality are better in buffaloes superovualted after the Ovsynch protocol.