author(s)
B Suresh Kumar, S Batabyal, UK Chattopadhyay, Atul Raj, U Vinod, Amit Banik, Bhattacharya S and M Yogendra

abstract
Hydatidosis is a neglected cyclo zoonotic disease impacting human and their livestock. In this study, Hydatid cysts from the liver and lungs of goats were collected from different slaughter houses and different butcher shops in and around Kolkata. From the Hydatid cysts, crude antigen was prepared and purification of antigen was done by gel filtration chromatography and analysis was performed by Sodium Dodecyl Sulphate Polyacrylamide gel (12.5%) electrophoresis (SDS-PAGE) under reduced conditions. The resolution of crude Goat hydatid cyst fluid antigen in SDS-PAGE revealed polypeptides with MW of 60 kDa, and 245 kDa. The purified antigen also showed the bands of polypeptides with MW of 60 kDa and 245 kDa. For purified antigens Double Immune Diffusion test was performed with antisera raised in healthy New Zealand White rabbits. The purified antigens were subjected to Western Blot technique to verify their immune reactivity with hyper immune sera which illustrated bands at 60 kDa and 245 kDa.