author(s)
Abhishek Kumar, Kamal Khilari, Ramji Singh, Ramesh Singh, SK Sachan and MK Yadav

abstract
An experiment was conducted to test the efficacy of metabolites (Culture filtrate) of bio-agents at two concentrations viz. 25%, 50% against larval motility and egg hatching of Meloidogyne graminicola casing root knot disease in rice under In vitro condition. The bioagents viz., Bacillus subtilis, Pseudomonas fluorescens, Paecilomyces lilacinus, and two isolates of Trichoderma (Trichoderma isolates S13 and Trichoderma isolates S7) tested in this study. All the tested bioagents significantly increased the larval mortality and reduced the egg hatching of Meloidogyne graminicola. Among the tested bioagents maximum (90.00%) larval mortality and minimum (48.33%) egg hatching was recorded at 50% concentration. While, at 25% concentration maximum (75.00%) larval mortality and minimum (55.00%) egg hatching was recorded in culture filtrate of Trichoderma isolate S13 after 72 hr of inoculation. Minimum 78.33% larval mortality and Maximum 58.33% egg hatching was recorded in culture filtrate of Paecilomyces lilacinus whereas, in case of untreated control 1.67% larval mortality and 88.33% egg hatching was recorded after 72 hour of J₂ inoculation.