author(s)
D Sengupta, SK Sheetal, CS Azad, Deepshikha Raj and Praveen Kumar

abstract
This work aimed to study the effect of administration of cloprostenol (PGF2α analog) on the efficacy of treatment with levofloxacin ornidazole combination in bitches with pyometra. Twelve bitches presented in the clinics with vaginal discharge were diagnosed for pyometra by ultrasonography, vaginal cytology and hemato-biochemistry. Bitches (n-12) were divided into two groups viz. LO (n=6) and LO+Clp (n=6). LO and LO+Clp group received a course of 2% levofloxacin and 4% ornidazole combination at the dose rate of 5 ml per 20 kg body weight twice daily for five days; in addition LO+Clp group received cloprostenol sodium at the dose rate of 5 µg/kg body weight sub-cutaneously thrice daily for five days. Serum progesterone level was measured on day 0, 1, 2 and 3 from the start of treatment (day 0). Vaginal cytology and hemato-biochemistry was repeated after 7 days from the start of the treatment (day 0). The progesterone level dropped sharply on day 1 in LO+Clp group compared to LO group (4.8±0.28 ng/ml and 1.8±0.07 ng/ml respectively; P<0.01). Clinical parameters like anorexia, vomition, malaise and neutrophils in vaginal cytology returned to normal levels in all six bitches of LO+Clp group 7 days after treatment but LO group showed partial recovery. Hemato-biochemistry revealed significant increase in hemoglobin and RBC count and significant decrease in WBC count, total protein, BUN, creatinine and ALP level in LO+Cl group compared to LO group. It can be inferred from the results that cloprostenol sodium, potentiates the action of levofloxacin probably by reducing the level of progesterone or by stimulating strong myometrial contraction and flushing of uterine contents.