author(s)
Joycee Jogi, Anju Nayak, PC Shukla, Amita Dubey, Ranvijay Singh, Ajay Rai, Poonam Shakya and Smita Bordoloi

abstract
The bacterium Pasteurella multocida is an opportunistic pathogen that is associated with the infections centering the animals such as bovines, swine, rabbits as well as poultry. It is widely known for its efficiency to cause Haemorrhagic Septicaemia, a complication characterized by high morbidity and mortality. In this study, P. multocida was cultured, isolated and was subjected to biochemical characterization for the purpose of identification. The DNA was then extracted from the cultured bacterium and the Molecular Characterization of P. multocida serotype B:2 strain was done by the employment of Polymerase chain reaction (PCR) targeting ompH using the specifically designed forward and reverse primers. The amplified products of desired gene were then visualized using 1% Agarose gel. The PCR yielded a 943 bp gene product. The molecular characterization of the bacterium P. multocida plays a very important role in demonstrating the efficiency of the P. multocida as a candidate for the production of vaccines.