author(s)
Santosh Kumar Verma, AK Srivastava, Neeraj Kumar Gangwar, Renu Singh and Shyama N Prabhu

abstract
For this study, 63 rats of either sex, aged 4 weeks were randomly divided into three groups comprising 21 animals in each. The animals of group I provided distilled water and served as control, group II and group III were provided Datura stramonium seed extract @ 1000 and @ 2000 mg/ kg body weight orally, respectively for 90 days. All the rats of both groups showed clinical symptoms of depression, reduced appetite, and dilatation of pupils (mydriasis), increased thirst, restlessness and excitation for a longer period. The values of Hb and PCV significantly decreased on day 30, 60 and 90 in group-III and on day 90 in group-II. TEC were significantly decreased at all intervals in group-III and 60^th and 90^th day in group-II. The TLC values showed highly significant leucopenia on 30, 60 and 90 day post feeding in rats of group-III and 60th and 90th days in group-II. There was highly significant neutrophilia at 30^th, 60^th and 90^th day in the rats of group-III and on 60^th and 90^th day of the experimentation period in the rats of group-II. There was highly significant lymphocytopenia at 30^th, 60^th and 90^th days of experimentation in the rats of group-III and on 90^th day of experimentation in the rats of group-II. The value of AST, bilirubin, urea, cholesterol and catalase significantly increased in rats of group-II on day 90 and in the rats of group-III on day 60 and 90. The plasma uric acid revealed highly significant increase in the mean value on day 90 in rats of group-II and III. The value of plasma creatinine and alkaline phosphatase was highly significant increase on day 60 and 90 in group-II and on day 30, 60 and 90 in the rats of group-III. The mean value of total proteins was highly significant reduced in the rats of group-III on day 60 and 90. However, the mean value of albumin was highly significant decrease on day 60 and 90 in group-II and on day 30, 60 and 90 in the rats of group-III.