author(s)
Rajesh Kumar S, Gupta ID, Raja KN, Virendra Kumar, Saravanan R and Kathiravan Periasamy

abstract
The present study was carried out to sequence characterize bovine S100A8 gene in Sahiwal (Bos indicus) and Karan Fries (Bos indicus X Bos taurus) cattle. S100A8 sequence data including 912 bp of coding DNA and 1kb of 5’ flanking region were generated in Sahiwal and Karan Fries cattle. The S100A8 coding DNA sequence of Sahiwal and Karan Fries cattle were found to have 100% identity with Bos taurus cattle, 99% with yak, 97% with buffalo and 85% with horse. A total of 18 putative sites were identified in 1kb region upstream to S100A8 gene and the location of most of these transcription factors except CP2 and NF kappa B were conserved in Sahiwal and Karan Fries cattle. The putative domain for binding CP2, found in Sahiwal cattle was absent in Karan Fries cattle while NF kappa B found in Karan Fries cattle was absent in Sahiwal.