Inflammation of the prostate gland in dogs is mostly suppurative in nature that may even lead to abscess. Loss of appetite, difficulty in urination, hematuria were the common manifestations, apart from other signs viz., tenesmus, dyschezia, pain and fever. Neutrophilic leukocytosis along with border line serum creatinine and blood urea nitrogen were the significant hemato-biochemical abnormalities. Asymmetric and nodular prostate gland was detected on per-rectal digital palpation and presence of distended, irregularly shaped prostate gland with mixed echogenic texture and multiple anechoeic foci in the gland were important ultrasonographic findings. Cultural examination of fine needle aspiration cytology of the sample revealed E. coli infection.