author(s)
Thirunavukkarasu SB, Pushpendra Kumar, Om Prakash, Amit Kumar and Ilayakumar K

abstract
The study was conducted to explore the polymorphism of CLA-DRB3 gene in Salem black goat by using PCR-RFLP technique. A region of exon 2 encompassing 285 bp fragment of DRB gene was amplified by polymerase chain reaction. The restriction digestion by BsaI revealed three genotypes AA (285 bp), AB (285/182/103 bp) and BB (182/103bp) with frequencies 0.930, 0.060 and 0.010, respectively and two alleles A and B with frequencies 0.96 and 0.04, respectively. The polymorphic information content (PIC) value and expected heterozygosity were 0.074 and 0.060, respectively which were low in both cases. The present study showed polymorphic nature of MHC Class II DRB exon2 Gene in Salem black goats at this locus and the frequencies of homozygote were greater than heterozygote.