author(s)
Aakanksha Rathore, Kaiser Parveen, Mohan Singh, Asit Jain, K Mukherjee, Deepti Kiran Barwa, Tripti Jain and Nishma Singh

abstract
The present study was carried out at College of Veterinary Science and A.H., Anjora, Durg (C.G.) to screen the Sahiwal animals and HF cross bred animals for BLAD and Bovine Citrullinemia genetic disorders by using PCR-RFLP method. The genomic DNA obtained from blood samples of 50 Sahiwal animals maintained at Bull Mother Experimental Farm, Dairy Unit, College of Veterinary Science and A.H, Anjora, Durg and 100 Holstein Friesian crossbred animals maintained at, ABIS farm, Durg were screened in the present study. An allele specific PCR-RFLP technique was applied to detect mutation in specific DNA target sequences and was standardized for Sahiwal and HF crossbred animals. The amplified products were purified and sequenced to confirm the change in nucleotide in the selected regions. None of the Sahiwal animals and HF crossbred animals was found to be carriers of BLAD and BC gene. Thus the gene frequency of normal allele is 1 and that of BLAD/BC gene is 0. The new methods of molecular genetics enable us to find the cause at gene level. Screening of all the animals in an organized farm, the breeding bulls and the prospective bulls is very important to check the spread of genetic defect genes in the population.