This investigation was conducted to study the effect of exogenous melatonin administration on testicular biometry of twelve sexually mature adult male Sirohi bucks during non-breeding season. Treated (T) animals (n=06) were administered single subcutaneous injection of melatonin. The Scrotal circumference (cm) and testicular volume (cm3) in control group [28.04±0.140, 167.90±4.439 (right) and 166.24±3.800 (left)] was comparable with that of treated group [28.91±0.193, 269.88±10.041 (right) and 267.53±9.568 (left)] for the first non-breeding season and for the second non-breeding season, control group [27.17±0.174, 120.05 ±5.843 (right) and 116.95 ±5.696 (left)] was comparable with that of treated group [32.50±0.302, 330.37 ±11.397 (right) and 329.22 ±10.846 (left)]the differences found significant for the both non-breeding seasons. Exogenous melatonin treatment significantly increased the scrotal circumference, testicular length, testicular width and testicular volume. In conclusion melatonin administration improved testicular biometry of Sirohi bucks during non-breeding season.