In vitro bioassay of Meloidogyne incognita juveniles against biocontrol agents
Nandeesha CV and Ravindra H
Biological control is considered as new efficient method that becomes widely used for controlling plant parasitic nematodes, as aim to decrease the extent of environment degradation and the effect of the excessive toxic nematicides. So, this study was done to investigate the role of four biocontrol agents against Meloidogyne incognita juveniles. In vitro nematicidal activity of bioagents against M. incognita juveniles was evaluated and juvenile mortality was recorded periodically at 24, 48 and 72 hrs. The mortality was found at 24 hrs and from then on, it increased with increase in time. Theresults showed that maximum mean mortality of juveniles was observed in Paecilomyces lilacinus (67.77%) culture followed by Trichoderma harzianum (54.89%), Pseudomonas fluorescens (50.00%) and the least mortality was observed in Bacillus subtilis (48.89%). The results of in vitro experiments indicated that, all tested biocontrol agents have a greatly significant effectiveness for juvenile mortality M. incognita.