Molecular detection of Salmonella Typhimurium and its antibiogram from marketed raw fish of inland fisheries sold in Aizawl, Mizoram, India
Devajani Deka, M Das and H Bayan
The study aimed to detect Salmonella spp in 100 numbers of raw fish samples from inland fisheries sold in local markets of Aizawl. Salmonella was isolated and presumptively identified by using conventional bacteriological method and the Salmonella serovars were further confirmed by detection of species specific 16S-rRNA gene and serotyping. The PCR positive Salmonella serovars were screened for the presence of virulence associated genes (inv A and stn) and antimicrobial susceptibility. From the 10 phenotypically positive Salmonella strains, 4 strains were confirmed as Salmonella by PCR (16Sr-RNA gene) and serotyped as Salmonella Typhimurium. All the 4 (100%) strains were subsequently positive for invA and stn genes. The antimicrobial sensitivity profile revealed that the Salmonella Typhimurium strains were sensitive to Amikacin, Gentamicin, Ofloxacine, Ceftazidime, Chloramphenicol and Imipenem and resistant to Tetracycline and Ciprofloxacin.
Devajani Deka, M Das, H Bayan. Molecular detection of Salmonella Typhimurium and its antibiogram from marketed raw fish of inland fisheries sold in Aizawl, Mizoram, India. J Entomol Zool Stud 2020;8(4):301-305.