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Journal of Entomology and Zoology Studies
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P-ISSN: 2349-6800, E-ISSN: 2320-7078

Journal of Entomology and Zoology Studies

2020, Vol. 8, Issue 3
Conventional and molecular detection of Salmonella typhimurium in sources of chicken origin and its antimicrobial resistance pattern in Mizoram (India)

Vanlalhruaii Hmar, Devajani Deka and Parimal Roychoudhury

The study aimed to detect Salmonella by cultural and molecular methods, its serotyping and antimicrobial sensitivity pattern in different samples of chicken sources from Mizoram. A total of 400 samples comprised of raw meat (50), internal organs (50), egg (50) and cloacal swab (50), each from Aizawl and Kolasib district were subjected to isolation and identification of Salmonella by conventional bacteriological methods which involved pre enrichment, enrichment, selective plating on agar media and biochemical tests. The presumptive Salmonella isolates were subjected to16S-rRNA gene detection and subsequently to serotyping and antimicrobial sensitivity test. A total of 27 (6.75%) Salmonella typhimurium serovars were confirmed by PCR and serotyping. Highest numbers of Salmonella were recovered in XLD agar with PCR conformity. Salmonella typhimurium was detected highest in internal organs followed by cloacal swab, egg and meat. The serovars showed 100 per cent sensitivity towards Imipenem and highest resistance to Tetracycline (92.59%).
Pages : 1270-1276 | 296 Views | 75 Downloads


Journal of Entomology and Zoology Studies Journal of Entomology and Zoology Studies
How to cite this article:
Vanlalhruaii Hmar, Devajani Deka, Parimal Roychoudhury. Conventional and molecular detection of Salmonella typhimurium in sources of chicken origin and its antimicrobial resistance pattern in Mizoram (India). J Entomol Zool Stud 2020;8(3):1270-1276.

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