Blood plasma biochemistry of broiler birds at slaughter age: Effect of anticoagulants and comparison with serum
SN Abedin, V Leela, NK Roy and V Ralte
The present study was conducted on 20 broiler birds that were slaughtered at 42 days of age and blood sample was collected in clot activator, Sodium Heparin and K3EDTA tubes to study blood biochemistry viz., estimation of albumin, triglycerides, creatinine, glucose, total protein, urea, chloride and inorganic phosphorous. Sodium Heparin recorded significantly (p<0.05) higher values for Albumin than K3EDTA.Triglyceride and Glucose showed significant (p<0.01) decrease in its levels in Sodium Heparin when compared to serum. Triglyceride level was highest in K3EDTA as compared to other anticoagulants. On the other hand, Glucose level was lowest in K3EDTA. Inorganic Phosphorous levels showed significant (p<0.05) increase in serum when compared against both the anticoagulants. Urea, total protein, creatinine and chloride levels were non-significant among the groups. In conclusion, heparinized plasma could be useful and preferable over K3EDTA for the measurement of biochemical parameters of the blood plasma of poultry birds viz., Albumin and Chloride. Phosphorous estimation being doubtful in heparinized plasma. K3EDTA causes little changes in the triglyceride levels. Glucose and total protein values seem to be less erroneous if measured in serum.
SN Abedin, V Leela, NK Roy, V Ralte. Blood plasma biochemistry of broiler birds at slaughter age: Effect of anticoagulants and comparison with serum. J Entomol Zool Stud 2020;8(3):728-731.