Pathomorphological, parasitological and molecular diagnosis of Babesia gibsoni in a dog
Shameem Ara Begum, Konkon Jyoti Dutta, Girin Hazarika, Pallabi Devi, Anupam Brahma and Lalit Deori
A 7 year old female German Shepered dog was presented with a primary complaint of inappetence and incoordination for 2 months. Physical examination revealed pale mucous membranes, enlarged pre-scapular lymph nodes, bilateral ocular discharges, pyrexia (41.2 °C), depression. History revealed that the dog was vaccinated and dewormed regularly. Following clinical examination, blood samples were collected for haematological, biochemical and parasitological analyses, and the results revealed the presence of intraerythrocytic Babesia parasites, normocytic and normochromic anaemia with extensive cellular damage. However, during the course of the investigation, the dog died. Post-mortem examination was carried out with appropriate consent. Gross examination revealed spleenomegaly, fatty change of liver, haemorrhage on the kidney and mucosal surface of stomach. Diffuse oedema with interstitial pneumonia in the lung, focal haemorrhage with cellular infiltration in the heart and coagulative necrosis, presence of urinary cast in the lumen of tubules, sub acute glomerulonephritis in the kidney were observed. Spleen revealed diffuse haemorrhage and stomach showed haemorrhage and congestion. Fatty changes associated with scattered haemoorhages were also recorded. Many of the observed clinicopathological alterations were consistent with chronic babesiosis.