This report describes a case of chronic atypical generalized dermatophilosis in a buffalo. Skin lesions were characterized by annular wheals and ulcerative lesions over neck, trunk, limbs and udder. Diagnosis was confirmed by cultural examination of skin scabs. Treatment response to procaine penicillin was transitory and complete recovery was achieved with two doses of long acting oxytetracycline within a month. The occurrence of infection was related to pregnancy.