author(s)
D Jain, RK Dhuria, T Sharma, Tara bothra and Umesh Kumar Prajapat

abstract
A 42 day feeding trial was carried out to investigate the effect of Tinospora cordifolia (at graded levels) or ascorbic acid alone and in combinations on Leucogram and H/L ratio using 360 one-day-old broiler chicks in 5×2 factorial experiment within a completely randomized design and divided into ten (10) dietary treatments groups (T₁-T₁₀) in triplicate of twelve chicks per replicate. The average temperature (31 ^oC) during the research trial was higher than the recommended normothermia zone i.e. 18-24 °C established for poultry birds, which indicated that poultry birds were in chronic heat stress. Improvement in serum albumin, serum globulin, total protein and A/G ratio of broilers was observed in the present trial due to supplementation of ascorbic acid or Tinospora cordifolia (geloi) alone and in combinations. Supplementation of ascorbic acid or Tinospora cordifolia (geloi) may be an indicator of immune response in broilers during chronic heat stress.