author(s)
PA Enbavalen, PK Ramkumar, S Rajagunalan, A Sundar and R Ramprabhu

abstract
A crossbred jersey cow presented with the history of chronic watery diarrhoea and was not responding to the treatment. Clinical examination revealed that the rectal mucosa was thickened. Differential diagnosis were a parasitic infection and Johne’s disease. Laboratory investigation revealed the presence of acid-fast organism in the rectal pinch sample. Further, the DNA extracted from the faecal sample and PCR targeting IS900 region of MAP was carried out to confirm the diagnosis.