Anaplasmosis is one of the most common infectious diseases of bovines causing economic losses to farmers due to decreased milk yield, weight loss, abortions and eventually death of the animal. A three year old female buffalo from a farm at Hyderabad was presented with a history of high fever, weakness, decreased milk yield, anorexia, apathy, weight loss and tick infestation. Clinically the animal showed pale ocular, buccal mucus membranes, rapid bounding pulse and lack of rumination. Complete blood picture (CBP) revealed decreased Total Erythrocyte Count (TEC), Hemoglobin concentration (Hb) and Packed Cell Volume (PCV). Blood smear examination revealed the presence of intraerythrocytic Anaplasma inclusions at the margins of the corpuscles concluding that the buffalo was suffering from Anaplasmosis. The faecal examination was negative for parasitic ova/oocysts. The animal was treated with oxytetracycline, antipyretics and hematinics for three consecutive days. Prompt improvement in health condition of the animal was noticed post treatment.