author(s)
Kotiya Anil S and Vadher KH

abstract
Shrimp culture activity was conducted at commercial pond at Datardi village, Rajula (Gujarat). Litopenaeus vannamei shrimp sample was collected treatment wise at final harvest taken out at 120 DOC. One kg ofshrimp sample were randomly collected from chilled tank for biochemical composition. Each treatment were implemented in triplicate. The present study was conducted to evaluate the effect of stocking density on L. vannamei with the emphasis on proximate, nutritional, amino acids and fatty acid composition. The proximate composition of L. vannamei was higher protein (%) in MT1 (30 nos/m²) treatment followed by crude fat (%), carbohydrate (%) and Ash (%). Comparing all treatment for major elements like calcium content was higher in MT6 (80 pc/m²) with 25.22±1.97 than potassium (K) in MT2 (40 pc/m²), sodium (Na) in MT2 (40 pc/m²) and magnesium (Mg) in MT6 (80 pc/m²) whereas minor elements predicted were iron (Fe) followed by copper (Cu), Zinc (Zn), Manganese (Mn) and Chromium (Cr). Totally 19 amino acids were detected, among these, arginine, histidine, isoleucine, threonine, leucine, methionine, phenylalanine, tryptophan, lysine and valine are essential amino acids and alanine, asparagine, aspartic acid, cysteine, glutamic acid, glycine, proline, serine and tyrosine are non-essential amino acids. In total essential amino acids recorded in MT4 (60 pc/m²) followed by MT3 (50 pc/m²), MT5 (70 pc/m²), MT6 (80 pc/m²), MT1 (30 pc/m²) and MT2 (40 pc/m²) whereas the total non essential amino acid (NEAAs) in treatment MT2 (40pc/m²) followed by MT1 (30pc/m²), MT6 (80 pc/m²), MT5 (70 pc/m²), MT3 (50 pc/m²) and low in MT4 (60 pc/m²).Total 16 fatty acid were detected in all treatment. The results indicated that L. vannamei shrimp has higher volume of SFA, PUFA, MUFA. The total unsaturated fatty acids (USFAs) was higher in treatment MT3 with (59.3 μg/g of FAME) followed by MT1 (58.67μg/g of FAME), MT4 (57.57 μg/g of FAME), MT2 (56.11 μg/g of FAME), MT6 (55.93μg/g of FAME) and MT5 (55.37μg/g of FAME). Comparing all the treatment there is clear evident that stocking density directly affect the proximate, nutritive, amino acid and fatty acid level in L. vannamei cultured shrimp, may be due to over crowding of shrimp stock or Alang ship breaking yard on one side and commercial port of pipava on another.