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Journal of Entomology and Zoology Studies
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P-ISSN: 2349-6800, E-ISSN: 2320-7078

Journal of Entomology and Zoology Studies

2020, Vol. 8, Issue 2
In-vitro evaluation of three herbal oils against larval stages of Lyperosia-Haematobia complex (Diptera: Muscidae)

JG Gudewar, BW Narladkar, HY Palampalle and SR Rajurkar

The present study was conducted to evaluate the efficacy of Neem (Azadirachta indica A. Juss.), Nilgiri (Eucalyptus globules Labill.) and Karanja (Pongamia glabra L.) crude oils against the field collected larvae of Lyperosia-Haematobia complex (Diptera: Muscidae). For the present experiment field collection of larvae was done from dung pits belonging to different buffalo shed nearby Parbhani city. In the laboratory few larvae were processed for identification using the standard keys. All the three oils in the study were tested at five different concentrations viz. 1, 1.5, 2, 3 and 5 percent aqueous solution and were sprayed on the faecal culture seeded with ten larvae per thirty gram of faeces in petridish. The oils were dissolved in the 5 ml of acetone and then in distilled water as per the desired concentration. Control group was maintained for each herbal oil as 10 larvae in 30 gram of faeces and sprayed with acetone mixed distilled water. All the trials were repeated thrice and average mortality count was recorded as assessment criteria for judging the efficacy of oils. The observations were taken till all the larvae in control group molted to pupal stage. The median lethal concentration (LC50) values of the Neem oil, Eucalyptus oil and Karanja oil against the larvae of Lyperosia-Haematobia complex were 3.63, 2.69 and 3.04% respectively. Thus the present experiment concluded that Neem oil, Eucalyptus oil and Karanja oil have potent action as knockdown effect against larval stages of Lyperosia-Haematobia complex and can be inducted in integrated pest management program after in-vivo evaluation.
Pages : 1169-1174 | 354 Views | 124 Downloads


Journal of Entomology and Zoology Studies Journal of Entomology and Zoology Studies
How to cite this article:
JG Gudewar, BW Narladkar, HY Palampalle, SR Rajurkar. In-vitro evaluation of three herbal oils against larval stages of Lyperosia-Haematobia complex (Diptera: Muscidae). J Entomol Zool Stud 2020;8(2):1169-1174.

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