Occurrence of thermophilic Campylobacter spp. in free-ranging chickens
Vivekanandhan R, ZB Dubal, Muralikrishna P, Arun Prince Milton A, Anukampa Kadwalia, Vengatachalam M and Priyadharshini R
Campylobacteriosis is one of the leading causes of foodborne bacterial gastroenteritis worldwide. An investigation was conducted to determine the occurrence of thermophilic Campylobacter spp. from free-ranging chickens. Chicken caecal samples were collected from fifty-four free-ranging chickens in Bareilly district, Uttar Pradesh. Samples were processed using modified charcoal cefoperazone deoxycholate agar (mCCDA) plates. They were then incubated at the microaerophilic conditions at 42 °C for 48 h and the presumptive colonies were subjected to Gram stain and other biochemical tests. Finally, suspected colonies were confirmed by targeting lipid gene lpxA performing a multiplex polymerase chain reaction. Campylobacter coli was isolated from three (5.55%) caecal samples and was confirmed with mPCR assay. It is highly essential to educate the free-ranging poultry rearers about the biosecurity measures, principles of food safety and hygienic handling of birds during rearing, slaughtering and cooking process to reduce the incidence of human campylobacteriosis.
Vivekanandhan R, ZB Dubal, Muralikrishna P, Arun Prince Milton A, Anukampa Kadwalia, Vengatachalam M, Priyadharshini R. Occurrence of thermophilic Campylobacter spp. in free-ranging chickens. J Entomol Zool Stud 2020;8(2):534-537.