author(s)
Bobby Shende and Suresh Masram

abstract
This study presents the oocyte development of Orthetrum sabina with the dynamics of histology and histochemistry. The ovary is panoistic type which contain many ovariole. Ovariole contain terminal filament, germarium, vitellarium. Five stages of oocytes were identified based on nature of cytoplasm, presence of germ vesicle, yolk granules and chorion. Spherical oval shaped oocyte I gradually increases in size and attained elliptical shaped oocyte V. Germinal vesicle is conspicuous in oocyte I and II but shifted towards one pole in oocyte III and completely disappear in oocyte IV. Chorion around plasma membrane manifested in oocyte IV. Proteins and carbohydrates entered in ooplasm from early stage while lipid appear late during oocyte development. Proteins, carbohydrates and lipid were demonstrated in oocytes by mercury bromophenol blue method, periodic acid Schiff method and Sudan black B method respectively.