author(s)
SD Kale, CH Pawshe, HS Birade, MV Ingawale, SG Deshmukh, SB Harkal and MB Ambalkar

abstract
In the present study effect of maturation media on early embryonic development of goat immature oocytes was conducted. The investigations were aimed to standardize the culture conditions for in vitro maturation, fertilization and in vitro embryo development of goat immature oocytes. The mean total number of oocytes recovered per ovary by slicing method was 10.79±1.00. All the collected oocytes were washed in oocytes washing media and transfer into two different maturation media TCM 199 (supplemented with 10% FCS, FSH and estradiol (1mg/ml) and commercial available maturation medium BO-IVM for 24hrs. After maturation the oocytes were fertilized in BO-IVF medium with 2×10⁶ sperm/ ml. After 24 hr of fertilization the presumptive zygote were transferred to in vitro culture medium BO-IVC. The embryo development rate was observed every 48 hr of incubation. The development rate was observed higher oocytes matured in commercial available BO-IVM medium than TCM 199 but there was not any significant difference between TCM 199 maturation medium and commercial available BO-IVM medium. From the present study it is concluded that the commercial available maturation medium i.e. BO-IVM showed higher per cent cleavage and embryonic development than TCM 199 for the culture condition in the embryonic development of the goat oocytes.