Six animals were administered Midazolam (3mg/kg) intravenously, 10 minutes later Propofol was given @3mg/kg body weight intravenously for induction of anaesthesia followed by immediate intubation; maintenance of anaesthesia was done under isoflurane. Anaesthetic combinations were compared by haematological and biochemical observations.

Haematological observations in the present study revealed that haemoglobin, packed cell volume and total erythrocyte count decreased significantly at maximum depth of anaesthesia in all the animals. Neutrophilia with relative lymphocytopenia were recorded during anaesthesia suggesting, certain amount of anaesthetic stress was produced by these combinations. In all the animals most of the biochemical parameters showed the changes, which were within the normal range, suggesting that the anaesthetic combinations used in the present study do not produce adverse effect on these parameters.