author(s)
Tejas C Shende, Vipul, Dr. Abhijit K Barate and Aakash Doiphode

abstract
In this study, partial exon 3 of TLR4 gene (T4CRBR2) of Indian crossbred cattle was characterized using PCR and bioinformatics tools. Analysis revealed that T4CRBR2 of Indian crossbred cattle had 100% identity with Bubalus bubalis, Bos taurus and Sahiwal breed sequences (both at nucleotide and amino acid level). At nucleotide level, high identity of T4CRBR2 (more than 99%) was also seen with Vechur cattle, Bos indicus x Bos taurus crossbreed cattle, Tharparkar cattle, bison and gayal sequences. At amino acid level 100% identity of T4CRBR2 was also seen with Vechur cattle, Bos indicus x Bos taurus crossbreed cattle, Tharparkar cattle, bison and gayal sequences. Nilgai T4CRBR2 had lowest identity compared to T4CRBR2 sequences of Indian crossbred cattle. In phylogenetic tree analysis T4CRBR2 of Indian crossbred cattle, Bubalus bubalis, Bos taurus and Sahiwal fall in one group, both at nucleotide and amino acid level.