author(s)
Ajay S Satbige, NA Patil, Baswaraj Awati and Sandeep H

abstract
The present study was conducted to detect the carrier status in Cattle. A total of 170 Cattle Urine samples were collected from Veterinary College Hospital with history of reproductive disorders, abortion, repeat breeding, and mastitis animals. All Urine samples were processed for extractions of DNA. Isolated DNA samples were amplified gene LipL32 of leptosira size 497bp of PCR. Out of 170 Urine samples 8 samples were found positive (4.70%) for leptosira spp. The result of present study showing evidence that there is a prevalence of leptospirosis infection in cattle. Carrier animals play a great role in maintenance and transmission of leptospirosis. PCR is an ideal test for detection of carriers.