Hypocalcemia in cows is commonly seen due to milk fever. It is also seen due to ephemeral fever and parenteral administration of aminoglycosides. In the present study, a rare occurrence of hypocalcemia during the post estrus period is reported. The study was conducted in and around anathi village of Hassan district and dasarigatta village of Tumkur district of Karnataka from 2015 to 2019. In this period, eight cows affected with hypocalcemia on the next day of estrus were treated. The clinical signs and response to calcium borogluconate therapy were similar to those observed in hypocalcemia during the periparturient period due to milk fever. The present study concluded that the probable reason for the development of hypocalcemia during the post estrus period may be due to elevation of blood estrogen level which might have interfered with the mobilization of calcium from calcium reserves to blood leading to hypocalcemia.