author(s)
Niranjan Soren, Rajashree Mishra, Kailash Singh Bisht, Iswar Anand Senapati and Prasana Kumar Rath

abstract
In the present study 209 samples of sheep and goats were collected from different farms and localities in and around Bhubaneswar, Odisha. Out of which 159 samples were collected from goats and 50 samples were collected from sheep. Both intestinal and fecal samples were collected and processed. A total of 19 samples were found positive indicating the prevalence of the Clostridium perfringens to be 9.09% in small ruminant population. Thioglycolate broth, Robertson’s cooked meat media (RCM), BHI and TPGY media were used in the present study. Morphological, cultural and bio-chemical characters of all the isolates of C. perfringens have been studied in detail. All the 19 isolates of C. perfringens were subjected to antibiotic sensitivity test and it was found that out of 15 antibiotic discs tested most of the local isolates were highly sensitive to sparfloxacin, erythromycin, enrofloxacin, doxycycline and amoxicillin. Growth characteristics of Clostridium perfringens on TPGY and BHI media revealed that BHI gives good growth in short time.