author(s)
Sumedha Bobade, K Vijayarani, KG Tirumurugaan, A Thangavelu and S Vairamuthu

abstract
Antimicrobial resistance (AMR) and emergence of multidrug resistant (MDR) in Staphylococcus aureus, is a major public health concern worldwide. S. aureus causes life-threatening diseases due to production of biofilm, a mechanism responsible for spread of multidrug resistance. Humans are infected with resistant foodborne bacteria via the food chain from reservoir animals. The aim of current study was to analyze the effect of chitosan coated iron oxide nanoparticles (IONP) on biofilm formation. To quantify biofilm production the Crystal Violet (CV) assay and Microtitre test assay (MTA) was used. Biofilm analysis by CV assay showed inhibition when incubated with Chitosan-coated nanoparticles. Biofilm inhibition was range from 66.17-99.25%, 68.63-98.86%, 91.41-97.66% at concentration of 500mg/mL, 1mg/mL and 4mg/mL respectively. Results indicate that chitosan coated IONP were effective in restraining biofilm formation, have potential to be developed as therapeutic agent where antibiotics are non-effective or in combination with antibiotics and against antibiotics resistant strains.