Screening potential microbes against whitefly (Bemisia tabaci (Gennadius)), the most important pest of cassava (Manihot esculenta Crantz)

ER Harish; AH Farhana; BG Sangeetha

Journal of Entomology and Zoology Studies

2019, Vol. 7, Issue 6

**Screening potential microbes against whitefly (Bemisia tabaci (Gennadius)), the most important pest of cassava (Manihot esculenta Crantz)**

Fig. 1: Adult pair of Bemisia tabaci

Fig. 2: Haemocytometer

Fig. 3: Spores as seen under Haemocytometer

Fig. 4: Bioassay of white fly

Fig. 5: Microscopic view of fungus grown on whitefly

Fig. 6: Bacterial bioassay in whitefly nymphs

Fig. 7: Bacterial bioassay in whitefly adult

PCR analysis of fungal isolates using ITS primers
(L: 1kb plus marker, lane 1- S<sub>3</sub>C<sub>1</sub>, lane 2- S<sub>1</sub>C<sub>1</sub>,
and Lane 4- S<sub>5</sub>C<sub>1</sub>)

Fig. 8: PCR analysis of fungal isolates using ITS primers (L: 1kb plus marker, lane 1- S₃C₁, lane 2- S₁C₁, and Lane 4- S₅C₁)

Fig. 9: PCR analysis of bacterial isolates using 16s primers (L: 1kb plus marker, lane 1- S1C4, lane 2- S6C3 and lane 3- S2C1)

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**Screening potential microbes against whitefly (Bemisia tabaci (Gennadius)), the most important pest of cassava (Manihot esculenta Crantz)**

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