The objective of present research work is to study follicular population and oocyte recovery in once a week and twice a week aspiration interval. A total 9 (Bos indicus) healthy animals were selected for trans vaginal follicular aspiration (TVFA) in every once weekly (every 7d; n=9) or twice weekly (every 3 or 4 d; n= 9). During each session, all visible follicles were aspirated using ultrasound guided trans vaginal follicular aspiration by using negative vacuum pressure of 90 mm Hg. Prior to the aspiration, follicular population were identified and measured and data recorded in three classes according to size (< 6mm, from 6 mm to 9 mm and > 9 mm). The COCs harvested from each follicle were counted and classified into 3 categories.

A total of 197 follicles were aspirated by during 60 aspiration sessions yielding 114 oocytes. There was no significant difference in average COCs/follicles recovered but recovery rate of oocyte was higher in twice week than once a week aspiration session (48.24% verses 71.08%). We concluded that follicular aspiration appears in twice a week with a higher number of harvestable follicles and more oocytes recovered than when done once a week.