Genetic diversity of whitefly, Bemisia tabaci population of Rajendranagar, Telangana, India
KP Manju, K Vijaya Lakshmi, Ch. V Durga Rani, B Sarath Babu and K Anitha
The genetic identification of whiteflies collected from the different fields of Rajendranagar, Hyderabad, Telangana, India during summer 2017 was done by SSR-PCR of the mitochondrial cytochrome oxidase I (mtCOI) gene. The results obtained from the present study revealed the amplification of mitochondrial cytochrome oxidase I (mtCOI) gene fragment using the primers (C1-J-2195 and L2-N-3014) produced Bemisia tabaci specific ~860bp band in all the samples of B. tabaci. MtCOI gene of this B. tabaci samples collected from Rajendranagar, Telangana were compared with other known sequences in GenBank and the test samples showed high level of nucleotide and amino acid identity with Asia I and Asia II-1 genetic groups. The phylogenetic tree constructed based on UPGMA (Unweighted Pair Group Method with Arithmetic Mean) analysis with 10 mtCO1 sequences of B. tabaci samples also revealedthat Rajendranagar B. tabaci population clustered with sequences corresponding to Asia-I and Asia II-1 genetic groups. So far there is no report on the presence of Asia II-1 genetic group from the Telangana state.
Fig. 1: Amplification of mt COI gene fragment using the universal primer (C1-J-2195 and L2-N-3014). L: DNA Ladder; (1-10) Bemisia tabaci samples collected from Rajendranagar, Telangana
Fig. 2: UPGMA (Unweighted Pair Group Method with Arithmetic Mean) phylogenetic tree of the mtCOI gene from Bemisia tabaci samples collected at Rajendranagar, Telangana. Bemisia atriplex (GU086362) was used as the outgroup
KP Manju, K Vijaya Lakshmi, Ch. V Durga Rani, B Sarath Babu, K Anitha. Genetic diversity of whitefly, Bemisia tabaci population of Rajendranagar, Telangana, India. J Entomol Zool Stud 2019;7(6):561-564.