Background: Hepatitis C virus is main cause of persistent liver disease and developments toward liver cirrhosis, later a main cause of Hepatocellular Carcinoma. Round about 4% of the people is diseased by this virus around the word.

Method: The data was collected from the period for December 2017 to October 2019. Over all 32,012 blood donors blood samples were screened with the help of ELISA kit (ARCHITECT i1000SR automated immunoassay analyzer chemiluminescent micro particle immunoassay).

Result: The frequency rate of HCV infection among blood donor using CMIA method at Mardan medical complex. From the total 32012 patients just 4587 cases were positive. The number of total male individual is 27079 and from total male individual just 4018 were positive which show (14.84%) ratio while the number of female individuals is 4933 and among total female case just 569 individual show positive result with percentage ratio is 11.53% which is quite less positive ratio than male positivity ratio.

Conclusions: we concluded that due to the blood transfusion and unawareness about HCV infection is the major cause of High prevalence of HCV. Another major cause is drinking and eating of unhygienic water and food also cause HCV.