Fig. 1: Evolutionary phylogenetic analysis of all candidates NPC2, OBPs, GOBPs and PBPs in hymenoptera species. Green color represents NPC2 and OBP clustered one clade, Brown color represents NPC2 and GOBP clustered one clade, Blue color represent NPC2 and PBP clustered one clade.

Fig. 2: Amino acid sequences alignment of representative NPC2 protein in hymenoptera was constructed with DNAMANN 8 software using multiple sequence alignment bases on full alignment methods. The red colors represent the consensus sequence of 6 Cysteine and 1 Proline

Fig. 3: Amino acid sequences alignment of olfactory proteins (OBPs, GOBPs, PBPs) in hymenoptera was constructed with DNAMANN 8 software using multiple sequence alignment base on full alignment methods. The representative olfactory proteins were selected as the results of nearest phylogeny. The red colors align represent the consensus sequence as 6 Cysteine

Fig. 4: Computational perdition of three dimensional structures comparative analysis between NPC2 and OBP1, OBP2, OBP3, OBP4 in M. cingulum was constructed by online software Swiss Models https://swissmodel.expasy.org/interactive