author(s)
Mini Kanchan, Udit Jain, Gourab Basak, Janardan K Yadav and Raghavendra Mishra

abstract
E. coli is known to be one of the most ubiquitous organisms. Consequently, its pathogenesis is also seen quite frequent in wide range of hosts but the intensity may differ enormously regardless of its serotype, quantity, virulence, immunity of the host etc. Recently Verotoxic E. coli (VTEC) has created a great havoc in both livestock and mankind and emerged as one of the major foodborne zoonosis which causes hindrance in economy. Therefore, diagnosis of pathogenic E. coli has become an utmost important as this helps in early detection of the pathogen. Besides, molecular analysis of E. coli genes leads to more specific and confirmed detection of the bacteria. Among the 70 different clinical samples from animals tested for E. coli, 29 showed positive whereas 2 of them showed positive for VTEC. 1 of them having stx₂ gene and another 1 showed stx₁+stx₂+hlyA gene combination. As a result, prevention remains the best option to control and uproot the zoonosis.