author(s)
Dina Nath Pandit and Anshu Sinha

abstract
This is an initial report on ecotoxicity of silver nanoparticles for Clarias batrachus (Linnaeus). The work was conducted by the use of static renewal method during 2018. In this experiment, 24hr-LC₅₀ value from Lorke and Enegide et al, technique was estimated severally 158.11 and 175.0 mg/L. A range of 96hr-LC₅₀ value of 158.33 to 191.67mg/L was calculated from Up-and-Down technique as a rough estimate. Moreover, from Behren-Karber, Miller and Tainter and Finney technique varied 96hr-LC₅₀ value was found 177.5, 171.6 and 171.4mg/L. Finally, Reed-Muench technique used to confirm 96hr-LC₅₀ dose and a median ideal dose was found 173.25mg/L. The findings suggest that silver nanoparticles are accumulated in aquatic bodies and distressing the physiological activities of Clarias batrachus. The findings inferred that this fish incorporates a ton of capability to resist silver nanoparticles toxicity compared to completely different fishes. Vary of tabulated safety level indicated that it's robust to settle on the acceptable dose of silver nanoparticles for this fish. However, on the premise of traditional toxicity varies; silver nanoparticles may even be treated as moderately cytotoxic substance to Clarias batrachus.