author(s)
M Venkateswarlu, K Sadasiva Rao, M Srinivas, V Devi Prasad and Thangamani A

abstract
The present study was undertaken to evaluate the changes in haematological and biochemical status in repeat breeding Graded Murrah buffaloes (n=60) in comparison with normal cyclical buffaloes (n=15). Blood samples were collected at observed estrus in both repeat breeders and normal cyclical buffaloes for estimation of haematological and biochemical parameters. Repeat breeder Graded Murrah buffaloes exhibited lowered levels of mean haemoglobin (8.61±0.20 gm%), Packed cell volume (35.21±0.99%) when compared to normal cyclic buffaloes. Further, biochemical parameters like mean serum glucose (48.26±2.83 mg/dl), calcium (7.21±0.18 mg/dl), phosphorus (4.44±0.12 mg/dl), total protein (6.57±0.09 g/dl) and cholesterol (85.57±1.90 mg/dl) concentration also showed lowered levels in repeat breeder buffaloes when compared to normal cyclical buffaloes. In conclusion, the decreased levels of biochemical parameters like calcium, phosphorus and cholesterol could be cause of repeat breeding in buffaloes.