author(s)
Value Debbarma, Sudip Sinha, L Kipjen Singh, BC Deka, RK Biswas, Rumi Saikia Borah, T Gogoi and Dibakar Baruah

abstract
A total of 22 pooled ejaculates comprising 94 ejaculates collected using artificial vagina from five adult Beetal bucks maintained at Goat Research Station, AAU, Burnihat were used to study the effect of extenders on the quality of buck semen preserved at 5 ºC. Each pooled ejaculate was split into three equal parts. The parts were extended using three extenders viz., Tris extender containing 20 percent egg yolk, Tris extender containing 1.5 percent soya lecithin and commercial Ovixcell extender by split-sample technique and then preserved in a refrigerator at 5 ºC. The extended semen samples were evaluated about two hours after collection before cooling to 5 °C and then at 24, 48, 72 and 96 hours of preservation at 5 °C for sperm motility, live sperm, intact acrosome and HOST-reacted sperm. The sperm motility and live sperm count in Tris extender containing 20 percent egg yolk were significantly (P<0.05) higher than Tris extender containing 1.5 percent soya lecithin and Ovixcell extender. A significant (P<0.05) decline was also observed in both sperm motility and live sperm count at each hour of preservation period in all the three extenders. The incidence of intact acrosome and HOST-reacted sperm in Tris extender containing 20 percent egg yolk was significantly (P<0.05) higher than the other two extenders and in Tris extender containing 1.5 percent soya lecithin than in Ovixcell extender. Both the acrosome and HOST-reacted sperm were significant (P<0.05) decline at each hour of the preservation period in all three extenders. It can be concluded that Tris-egg yolk extender is superior to Tris-soya lecithin and Ovixcell extenders for the preservation of buck semen at 5 °C.