author(s)
Amit Kumar, Sujata Pandita and Anand Laxmi N

abstract
Prostasomes are extracellular vesicles released by prostate gland during ejaculation. They can fuse with sperms, and improve different sperm functional parameters. Present study aimed to investigate prostasomes effects on in vitro growth of Bacillus megaterium bacteria, as well as, on motility and viability of Karan Fries and Sahiwal bulls’ spermatozoa. Bacteria was incubated with prostasomes for overnight in Luria-Bertani broth, and subsequently inoculated into culture plates from the broth. Spermatozoa was obtained by swim up technique from fresh and cryopreserved-thawed semen of Karan Fries and Sahiwal bulls and incubated with prostasomes (1mg/ml). Prostasomes supplementation revealed inhibitory effect on Bacillus megaterium growth, and beneficial effect on sperm motility and viability in case of fresh and cryopreserved-thawed Karan Fries and Sahiwal bulls’ spermatozoa. Present investigation suggests prostasomes supplementation can be advantageous towards the improvement of quality of fresh and cryopreserved semen.