author(s)
Supriya Das, SK Panda, AP Acharya, UK Mishra, AK Kundu, BN Mohanty and I Ali

abstract
Post-mortem interval (PMI) is the time that has elapsed since the animal has died. Estimation of post-mortem interval (PMI) is very important for forensic cases or in veterolegal investigations. The present study was conducted for estimation of post-mortem interval through tissue and serum enzymatic changes after death. Tissue enzymatic changes were studied for ALT and AST in heart as well in liver tissue of goat. Alternation of serum enzymes i.e, AST, ALT, LDH and Alkaline phosphatase were studied after death at different time interval. Tissue enzymatic study of liver & heart revealed gradual increase in AST & ALT values at 6 & 12 hrs. Serum enzymes like AST, ALT, LDH and alkaline phosphatase increased with increase in time after death upto 24 hrs of post death. The changes in these enzymatic values with time passed since death can be used in estimation of post-mortem interval.