author(s)
MK Kumhar, Sunita, SK Kashyap, Rajani Joshi and BN Shringi

abstract
Brucella is one of the most important causative agent in bovine abortion in dairy cattle and have significance zoonotic importance in developing countries. The protocol was tested on different 168 blood samples (paired=80,unpaired=8) from HF cross dairy cattle, with a history of abortion, retention of placenta or repeat breeding or any combination of these and known to be not vaccinated, in and around Bikaner city. Out of all blood samples, 26 (32.5%) were found positive which were subjected to RBPT using Rose Bengal colored antigen. Preventive and control measures should be implemented and pursued more strictly to reduce and/or eradicate brucellosis.