author(s)
UM Tumlam, VC Ingle, PA Tembhurne, NV Kurkure and SP Chaudhari

abstract
Bovine fecal samples of cattle calves (180), buffaloes calves (31) between the age group of 0-1yrs were collected from diarrheic animals from different regions of Maharashtra during the year 2016-17. Of them a total of 58 fecal samples comprising of 48 (cattle calves), 10 (buffaloes calves), screened for the presence of rotavirus by rapid Rota virus Ag detection Biochrom kit & RNA-PAGE. The rotavirus was detected in 4 out of 58 bovine fecal samples by rapid Rota virus Ag detection and one sample of cattle calf found positive for the RNA-PAGE assay. The bovine rotaviruses were positive for electrophoretic migration pattern of 11 segments with a typical pattern of 4:2:3:2. In a long run, segment 10^th and 11^th of all bovine rotavirus migrated faster as compared to short run electropherotype, while no sample from buffalo calves was found positive for both the test. RNA-PAGE positive sample of calf was subjected to RT–PCR of VP7 & VP4 gene which yielded amplicon of 1011& 864bp RT-PCR targeting group specific VP7 & VP4gene confirmed Group A rotavirus.