author(s)
UM Tumlam, VC Ingle, Dhruv Desai and SR Warke

abstract
Rotavirus has been registered as one of the common and worldwide prevalent causative agent of enteric illness in newborn of most mammals and poultry species. The infection brings about severe symptoms of diarrhea in lambs, pigs and calves. In the present study out of 58 cattle & buffaloes calves fecal sample 04 (6.89%), 50 human infants stool samples 18 (36.00%), 71 piglets fecal samples 07(9.85%) were screened by rapid antigen detection kit. It was observed that out of 4 faecal samples from calves, 1 sample and out of 18 human stool samples, 07 samples out of 7 piglets 3 samples were found positive for RNA-PAGE. Representative PAGE positive fecal and stool samples, were selected for RT-PCR. The VP4 & VP7 genes of one calve sample were successfully amplified by RT-PCR with expected size of1011bp & 864bp. out of seven only six human infants samples amplify for VP7 gene, 3 out of 7 human infant sample amplify for VP4 gene and 3 out of 3 piglet samples amplify for VP4 & VP7 gene with the expected size of 1062 bp & 876 bp were confirmed positive for Rotavirus infection. One sample from calve and one from human infants, one from piglets were sent for sequencing. The phylogenetic analysis was carried out and it was confirmed that the positive samples were showing relatedness with the Rotavirus sequences taken from genebank and after sequence analysis, it was found that the piglets positive sample showed its relation with human rotavirus. This is indicative of trans-species transmission of rotavirus can occur. This work indicates that the migration of rotavirus throughout the country is possible.