author(s)
Amit Kumar, Sudarshan Kumar, Madhu Shivhare, Ranjit Aich, Vinita and Shailendra

abstract
The present study was conducted on 36 crossbred cows with history of repeat insemination at different intervals, randomly categorized in infectious and non-infectious groups on basis of positive or negative reaction to Whiteside test belonging to the Dairy farm of College of Veterinary Science and Animal Husbandry, Mhow and clinical cases of progressive farmers brought for artificial insemination to Teaching Veterinary Clinical Complex and at the doorstep of farmers in nearby villages. These selected animals divided into 6 groups viz. GnRH analogue (Gp I), GnRH + HCG (Gp II), HCG alone (Gp III), HCG + GnRH (Gp IV), Progesterone (Gp V) and Control Group (Gp VI). The cervico-vaginal mucus samples were collected from the animals at oestrus and were immediately used for physical parameter analysis. The difference in spinnbarkeit values between hormonal treated repeat breeding and placebo treated repeat breeding cross bred cows were observed to be highly significant (P<0.01). The overall mean pH value found in the non-pregnant cows was 8.72 ± 0.22 and the difference was statistically non-significant. The overall mean values of vaginal electrical impedance (ohms) of cervico-vaginal mucus in overall conceived crossbred cows were highly significant (P<0.01) at 0, 12^th and 45^th day, whilst in all non-conceived groups, it was highly significant (P<0.01) at day 0 and 12^th day and non-significant at day 45^th.