author(s)
Uday Bikash Oraon, Lourembam Sanajaoba Singh and Jayanta Tarafdar

abstract
In our present investigation the leaf curl disease on chilli was observed up to an extent of 18-85% at different regions of Gangetic alluvial zone of West Bengal. The disease was successfully transmitted by whitefly (Bemisia tabaci), on experimental plants. The total DNA was extracted from infected leaf samples and PCR was carried out using a pair of begomovirus specific degenerate primers (SPG1/SPG2). The replication associated protein gene fragment (Rep; AC1) of 920 bp were amplified from naturally begomovirus infected and experimentally vector transmitted plants. NCBI blast analysis of partial nucleotide sequences of DNA-A revealed that Bongaon - North 24 Parganas - WB (Accession no. LT622253) and Dhatrigram - Burdwan - WB (Accession no: LT622254) begomovirus isolates shown 91% and 95% nucleotide sequence identity with Pepper leaf curl Bangladesh virus (Accession no: AF314531 & HM007111) respectively.