author(s)
Sahrish Khan, Junaid Ijaz, Tayyaba Zahra, Muhammad Khizer Hayat, Muhammad Hashim Raza and Kumail Abbas

abstract
Visually identification of sex is difficult in Class Aves because birds are monomorphic to identify sex on the basis of phenotypic characteristics was impossible. The Birds grown-up females seem indistinguishable to the males in half of the world. This issue can hinder by both human-helped reproducing of winged animals and transformative examinations. DNA-based sex distinguishing proof gives an answer. Researchers depict a test in light of CHD (chromo-helicase-DNA) gene present on the avian sex chromosomes of all bird species with the conceivable special case of the ratites. The CHD-W is arranged on the W chromosome; it is for females. On the Z chromosome CHD-Z, is found, it occurs in both sexes (female, ZW; male, ZZ). In this study, sex of Rock Pigeons (Columba livia) was identified. Three birds were obtained from local market. DNA was extracted from the tissue samples. DNA was quantified by Nano Drop. Gene was amplified on a PCR using sex specific primers. Amplicons were analysed on agarosegel. It intensified homologous segments of the two genes and includes intron's lengths which normally vary. When PCR products were revealed on gel electrophoresis single Z-band was obtained which indicates that one sample was male birds and two were female having double bands. It was the first study in Pakistan to utilize CHD gene for sex identification in rock pigeon which proved the validity of the genetic marker for this species. This study can pave a way to build effective conservation programs and successful breeding strategies.